Growth Dynamics Of E. Coli In Varying Concentrations Of Nutrient Broths, PH, And In The Presence Of An Antibiotic

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producing 89,3 01
cells/ml; glucose producing 411,951 cells/mL; lactose producing 477,441 cells/mL
and finally pH 6.0 producing 579,557cells/mL. The remaining four media, after
calculations, produced cell counts greater than the control: 2X with 1,087,009
cells/mL; 0.5X with 2,205,026 cells/mL; pH 8 with 3,583,750 cells/mL and finally
pH 7.0 with 8,090,325 cells/mL. From these results the conclusion can be made
that the environment is a controlling factor in the growth dynamics of E. coli.
This was found through the regulation of pH and nutrient concentrations. In the
presence of the drug/antibiotic, chloramphenicol, cell growth was minimal.

Introduction

E. coli grows and divides through asexual reproduction. Growth will continue
until all nutrients are depleted and the wastes rise to a toxic level. This is
demonstrated by the Log of the number of cells per unit volume versus Time
growth curve. This growth curve consists of four phases: Lag, Exponential,
Stationary, and ...

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The k value can be easily derived by knowing the number of cells in a
exponentially growing population at two different times. K is determined using
the equation k=(ln N-ln N0 )/t, where ln N is the natural log of the number of
cells at some time t, ln N0 is the natural log of the initial number of cells
and t is time. This equation allows one to calculate the numbers of cells in a
culture at any given time. The reciprocal of k is the mean doubling time, in
other words, the time required for the population to double, usually expressed
as cells per unit volume. (Edick 61-62)
Temperature is the most influential factor of growth in bacteria. The
optimal temperature of E. coli is 37C, ...

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