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Bio Lab Write Up - College Essays

Bio Lab Write Up

Abstract

This report is a study of various light intensities of 0-watts, 75-watts, and 100-watts of light intensities on the light reaction in photosynthesis of spinach chloroplasts suspended in 0.5 M sucrose. During the experiment DCPIP was used in the chloroplasts in place of NADPH in the light reaction to be able to monitor the rate at which the photosynthesis occurred. Due to the dye indicator, DCPIP, being reduced and turning from a blue solution to a clear solution, a spectrometer is used to measure the absorbance of the solution. The rate at which the DCPIP becomes reduced is directly proportional to the rate of the light reaction in photosynthesis. The solution showed to respond ...

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reaction where the ATP is produced from the light energy and the light-dependent reaction where the ATP is used to produce the glucose from the carbon dioxide.

The electron transport system is used during the light dependent to generate ATP and NADPH. The rate at which the electrons flow in regards to the light intensity can be measured by studying the flow of electrons from chlorophyll to an indicator dye. The indicator dye used in this experiment is 2,6-dichlorophenol-indolphenol (DCPIP), which is blue in its oxidized form. As the DCPIP accepts electrons from the electron transport system during the light reaction, the DCPIP becomes reduced and becomes colorless. The rate in which the DCPIP becomes reduced and becomes colorless is directly proportional to the rate of the light reaction in photosynthesis.

Materials and Methods

Place a test tube rack 30 centimeters away from a lamp containing a 75-watt bulb. Place a filled 1000 milliliter beaker with water in ...

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Bio Lab Write Up. (2011, March 27). Retrieved January 22, 2025, from http://www.essayworld.com/essays/Bio-Lab-Write-Up/96912

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PAPER DETAILS

Added: 3/27/2011 07:06:09 PM
Submitted By: msballer22
Category: Science & Nature
Type: Premium Paper
Words: 603
Pages: 3

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