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Fluorescence Study Of Dyes - College Term Papers

Fluorescence Study Of Dyes

1. Introduction
Coumarin 1 and Sodium fluorescein are two dyes which absorb and emit light in the visible region. By using a spectrophotometer we are recording an absorption spectrum and then determining the molar decadic absorption coefficients, which will be used later to interpret and analyse the fluorescence spectra.
For fluorescence spectra, there are two kinds, the excitation and the emission spectrum.
The excitation spectrum is obtained by measuring the intensity of the emission as the excitation wavelength is altered by scanning a monochromator. The emission spectrum is obtained by measuring the emission intensity as a function of wavelength for excitation at a fixed wavelength.
...

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distance at which decay and energy transfer are equally probable, as well as prove that Stern-Volmer's law and Förster's theory are obeyed.

2. Results

2.1. Electronic Absorption Spectra

We made up stock solutions for both salts and diluted them down to use in the UV/vis spectrometer.

Coumarin 1

C = 8 x10-5 mol dm -3
We obtained a spectrum with a maximum at 376.5nm at an absorbance of 1.4573, using Beer-Lambert's law, we deduced the molar decadic absorption coefficients.

Sodium fluorescein

C = 2.425 x10-5 mol dm -3
Here max was 500.5 nm at an absorbance of 2.0922

Summary of the results obtained.

Dye
max (nm)
max (m2 mol-1)
(m2 mol-1)
' (m2 mol-1)

D (Coumarin)
376.5
1.82 x 109
---
1.25 x 106

A (Fluorescein)
500.5
5.17 x 107
1.41 x 106
8 x 106

2.2. Fluorimetry

a) Perylene standard

/nm I

Emission Spectrum 438 4.063 EX = 434nm
467 2.620

Excitation Spectrum 410 2.755 EX = ...

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PAPER DETAILS

Added: 2/19/2006 02:49:48 AM
Category: Science & Nature
Type: Free Paper
Words: 815
Pages: 3

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