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Experimental Protein - Example Papers

Experimental Protein


Proteins are the macromolecules that are responsible for most of the bodily functions. By investigating an individual protein, one can be able to understand the functions and structure of an organism. Before this can be done, protein has to be separated from cell components. Using the methods of centrifugation and gel electrophoresis, not only a protein can be separated from cellular components but also from other proteins. An experiment was designed to separate protein contained in liver tissue of a cow based on it's molecular weight. From the centrifugation, soluble protein concentration extracted for the electrophoresis. Dilution was prepared of this concentrated extract; positive ...

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with huge molecular weight did not travel far from the gel while low molecular weight proteins traveled long distance . Overall banding contrast was compared between same sample with different transferred volumes. The protein bands of ADH were closely related to some of the bands of liver protein extract. ADH, which is derived from horse contains some of the protein that are related to the proteins found on cow's liver cells. Closely ranged Rf value is key to similarities that exists between protein bands based on their molecular weight. The results of these experiments were compared with research group with similar interests; the results and conclusion drawn were closely correlated.
Introduction
Our body consists of various types of macromolecules, which are the fundamental functional unit of life. These macromolecules are responsible for most of all of the cell functions. They include molecules such as carbohydrates (glycogen, starch, cellulose, chitin), lipids ...

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of a protein(1,2). Proteins are microscopic molecules that consists of various molecular weight, shape, and charge. One cannot study the functions and structural variations in an intact cells(2).
The protein must be separated using proper methods and techniques in able observe and calculate special properties. Proteins further can be targeted using antibody (6) The separated proteins are used as procedural method to purify a particular protein. The techniques used for protein separation is important in able to further investigate the native structure, and functions of a protein (2). The procedures of protein separation is a trivial procedure for laboratory workers.
Prior to ...

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PAPER DETAILS
Added: 3/10/2007 05:55:05 PM
Category: Science & Nature
Type: Free Paper
Words: 3276
Pages: 12

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